Abstract
Sheep erythrocytes carrying 100–200 SAC′ la,4 (rabbit antibody and guinea pig complement) were labelled with 86Rb and treated with varying dilutions of guinea pig C′2 at 30°C. At the peak of SAC't' la, 4,2a generation, C′-EDTA was added and kinetic sampling was begun by means of a simple filtration technique for rapid separation of cells from medium. Release of 86Rb⨥ started after a lag phase of about 0·5–1·0 min, and followed first order kinetics during a prolytic phase lasting 1–15 min, depending on C′2 mulitplicity. Cell lysis then ensued, with first-order release of hemoglobin (Hb). The kinetics of 86Rb⨥ and Hb release form the control populations. A physiological interpreration of the controls is suggested.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
