Abstract
The binuclear copper active site of Carcinus hemocyanin has been reconstituted by incubating apohemocyanin with Cu(I) in the presence of Br - ions. At constant Cu(I) concentration the kinetics of reconstitution depends on both pH and Br - concentration. The process is faster at pH 6.0 than at pH 7.0 and in both cases the reaction is accelerated by increasing Br - concentration from 0.1 M to 0.4 M. At pH 6.0 a time-dependent inactivation of the O 2-binding properties of reconstituted hemocyanin is observed. This effect is attributed to a perturbation in the active site microenvironment caused by unspecifically bound copper. Br - ions show a protective effect probably by chelating excess metal.
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