Abstract
The binuclear copper active site of Carcinus hemocyanin has been reconstituted by incubating apohemocyanin with Cu(I) in the presence of Br - ions. At constant Cu(I) concentration the kinetics of reconstitution depends on both pH and Br - concentration. The process is faster at pH 6.0 than at pH 7.0 and in both cases the reaction is accelerated by increasing Br - concentration from 0.1 M to 0.4 M. At pH 6.0 a time-dependent inactivation of the O 2-binding properties of reconstituted hemocyanin is observed. This effect is attributed to a perturbation in the active site microenvironment caused by unspecifically bound copper. Br - ions show a protective effect probably by chelating excess metal.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.