The kinetics of photoinactivation and long-term photosensitivity of pigmented and nonpigmented strains of the smut fungusUstilago violacea

Abstract

White, pink, pumpkin, and yellow strains ofUstilago violacea containing high and low levels of cytochrome c and various carotenes were subjected to high light intensities to characterize the kinetics of photoinactivation. Additionally, these strains were grown at two light intensities to characterize their long-term resistance to photoinactivation. The orange strain 2.D37291S and yellow strain 1.C2y had the highest carotene contents and were the most light resistant in the kinetics and growth experiments. The pink strains 2.C425, AB278a-1, and 1.C421 accumulated cytochrome c as well as carotenes. These strains were slightly more photosensitive than the yellow or orange strains in the kinetics experiment but were much more sensitive in the growth experiment. The phytoene-containing white strain 2.C419, which contains a small amount of cytochrome c, had a high level of resistance to light in the kinetics and growth experiments. The carotene-less strains were most sensitive in the kinetics experiment but not in the growth experiment. The overall photosensitivity ofU. violacea strains is related to the carotene and cytochrome c contents.