The kinesin-1 regulation mechanism of MAP7

Abstract

Kinesin-1 is an essential molecular motor that performs microtubule (MT) based transport of cargos in the cell. MT associated protein 7 (MAP7) is a required cofactor to kinesin-1 shown to promote motor processivity and is responsible for cargo transport and organelle positioning. Using in vitro reconstitution methods, protein engineering, and single molecule imaging, we dissected the mechanism by which MAP7 influences kinesin activity. MAP7 recruits kinesin-1 to the MT through a kinesin-binding domain in its intrinsically disordered projection region.