Abstract
SummaryBioinformatics applications increasingly rely on ad hoc disk storage of k-mer sets, e.g. for de Bruijn graphs or alignment indexes. Here, we introduce the K-mer File Format as a general lossless framework for storing and manipulating k-mer sets, realizing space savings of 3–5× compared to other formats, and bringing interoperability across tools.Availability and implementationFormat specification, C++/Rust API, tools: https://github.com/Kmer-File-Format/.Supplementary information Supplementary data are available at Bioinformatics online.
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