The Isolation of Termofil Amilolitik Bacterium and Activity Test of Harsh Extract Amylase Enzyme From The Hot Spring at Jailolo Bay in North Maluku

Abstract

Amylase is a hydrolase group enzyme used in many industrial areas which operate on high temperature. Amylase can be yielded from various organisms. The most common producer of Amylase is Bacterium. Bacterium can live in many extreme environment like hot temperature environment. One of the sources of hot water is Jailolo Bay in North Maluku, on that account it requires to conduct the isolation of thermophile amolytic bacteria from the hot water source of Jailolo Bay in North Maluku Moluccas North and test its amylase activity. Water sample used in this research was taken come from the hot water source with temperature of 75°C and 6,1 water pH. This research was done by isolating the bacteria, by packing 1 mL of the water sample into an add-on medium. Then 1 mL microbe culture was disseminated to a jelly medium and incubated for two days at the temperature of 40 °C, 50 °C and 60 °C. The resulting Bacterium colony was then processed qualitatively into the amolytic bacterium test in the jelly medium containing 1% sago extract with Iodium. Isolates yielding wide, transparent zone in each incubation temperature were identified. Identifying the bacteria was conducted with colony morphology test, biochemical test, and the coloration of Gram. The α-amylase enzyme activity test was relied on the amount of reduction sugar (maltose) which was produced by the extract hydrolysis with DNS method using maltose as standard. The result of the research shows that the source of hot water in Jailolo Bay, North Maluku has potency to yield thermophile amolytic bacteria. The amount of isolates bacteria obtained was 68 isolates from the temperatures of 40°C, 50°C and 60°C each resulting 36, 18 and 14 isolates bacteria. Three chosen bacterium isolates (big transparent zone) from each incubation temperature are isolates 51Y222 ( 40 °C), isolates 52B23 ( 50ºC) and 52YA ( 60ºC) which owns each transparent zone of 9,11 cm; 9,13 cm and 9,20 cm. The microscopic characterization result of Gram coloration indicates that those three isolates are included as the positive Gram bacteria and in form of bacillus. Based on the colony morphology observation macroscopically, microscopically and according to the biochemical test result, isolates 51Y222, 52B23 and 52YA represent bacterium of Bacillus sp. Amolytic enzyme activity of 51Y222, 52B23 and 52YA is each 157,75 mU / mL; 124,07 mU / mL and 1315,61 mU / mL