The isolation of synaptosomes from the brain of a teleost fish, Centriopristes striatus.

Abstract

Abstract— The brain of a typical teleost fish, sea bass (Centrioprisles striatus), yielded subcellular fractions similar to those previously isolated from mammalian brain upon homogenization and differential and density gradient centrifuging. In particular, a well‐defined synaptosomal fraction accounting for most of the bound acetylcholine of the tissue was obtained, although possibly because of the large amount of myelin in fish brain this and other fractions were not as homogeneous as the corresponding ones from guinea pig cerebral cortex. No difficulty was experienced in adapting the methods to the relatively small amounts of tissue (about 200 mg) obtainable from each fish. The isolation of synaptosomes in good yield indicates that teleost central presynaptic terminals have essentially the same physical properties as those of higher forms, permitting detachment and sealing under conditions of moderate liquid shear.