Abstract
The sea nettle (Chrysaora quinquecirrha) kills its prey with potent venom, which is contained in nematocysts found on its tentacles. This venom is a complex mixture of enzymes and toxic agents. The venom is thermolabile and may be degraded by the proteolytic agents it contains. The purification of the venom's lethal factors has been hindered by low yields of pharmacological activity upon chromatography as well as the large volumes required to assess lethality in mice. This chapter describes a technique for the isolation of sea nettle lethal factor I via immobilized monoclonal antibodies. In the technique, monoclonal antibodies (MAB) were prepared using the crude venom for immunization.
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