Abstract
THE ISOLATION OF PYRIMIDINE DEOXYRIBONUCLEOTIDES FROM THE ACID-SOLUBLE EXTRACT OF THYMUS
Highlights
The present study shows that some possible precursors of DNA, namely the mono, di, and triphosphates of thymidine and deoxycytidine, occur in thymus
Chromatographic Separation of Acid-Soluble Componentsof Calf Thymus -A typical chromatogram (Fig. 1) shows many similarities to those published for other tissue and cell extracts [9, 23, 24]
Deoxyribose content was somewhat low (0.79 pmole per micromole of cytidine compound) and the ratio of the absorption minimum to maximum was somewhat high, the sample was rechromatographed by ion exchange with formic acid and a single peak was obtained
Summary
Preparation of Acid-Soluble Extract-Fresh thymus, obtained at a local slaughterhouse within a few seconds after a calf was killed, was immediately cut into several pieces and thoroughly mixed with crushed ice. The chilled tissue was cut into small pieces with scissors and 60 gm. Batches were minced in 4 volumes of cold 0.6 RTperchloric acid at O-5” for 1 minute in a Waring blendor. Preliminary reports have appeared [1, 2]
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