Abstract
An improved method for the isolation of translatable mRNA from the white rot fungus Phanerochaete chrysosporium has been devised. Polacrylamide gel electrophoretic analysis of the in vitro translation products visualises polypeptides that have molecular weights up to and in excess of 200 000, demonstrating that no significant degradation of the mRNA occurs during its isolation. It has also been demonstrated that the RNA extraction method used with Phanerochaete can be successfully extended for use on another unrelated basidiomycete, Coprinus cinereus. The mRNA populations isolated from non-ligninolytic and ligninolytic cultures of Phanerochaete chrysosporium show several differences when compared by the electrophoretic analysis of their in vitro translation products.
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