Abstract
MRNA was prepared from first trimester placentae and translated in a wheat germ cell-free system. A major synthesized product had an apparent molecular weight of about 16,000. Tryptic fingerprint analysis revealed that this protein contained peptides overlapping with peptides from authentic alppha subunit of hCG. Since the protein portion of alpha has a molecular weight of 10,600, the data suggest the synthesis of a precursor. No synthesis of the beta subunit was detected. With first trimester RNA, 4–5 times more of the alpha protein was synthesized than with term RNA. Thus, it appears that the larger amounts of hCG-alpha produced in first trimester placenta is due to an increased proportion of the corresponding mRNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
