The isolation of large molecular mass procyanidins from grain sorghum and their interaction with [ 14c]methyl bovine serum albumins

Abstract

1. 1. Three protein-free procyanidin fractions with molecular masses of 4000, 8000 and 10,000–50,000 were isolated from a bird resistant sorghum grain. 2. 2. The interaction between the largest procyanidin and 88 or 1% methylated bovine serum albumin ( 14C labelled) is stronger with the more methylated protein over a range of 0–2.75 ionic stength at pH 5. This indicates the involvement of apolar forces throughout the range though apolar forces were more pronounced from 0.7–2.75 ionic strength. 3. 3. In the interaction between procyanidins of different molecular masses and 88% 14C-methylated bovine serum albumin the smaller procyanidins exhibited a more pronounced sigmoidal precipitation pattern than the larger with an increase in their concentrations.