Abstract
SUMMARY: Haemophilus pertussis cells contain a relatively large amount of deoxy-ribonucleic acid which may be extracted from the organisms with 2% sodium cholate. A method is described for the isolation of deoxyribonucleic acid from the mechanically disintegrated organisms, which yields an apparently undegraded preparation free from ribonuclcic acid and protein, but containing a polysaccharide component, probably in combination with the nucleic acid. This finding gives further evidence of the differences in biological properties of virulent and avirulent strains such as agglutinability by type-specific serum. precipitability by aluminium phosphate, and solubility in bile and sodium hydroxide.
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