The Isolation and Molecular Identification of Bacterial and Fungal Species from Potato Fields of Indore District of Madhya Pradesh

Abstract

The microorganisms of rhizosphere play an important role in development of healthy plant. The rhizosphere is the specific fine region of soil that is directly in contact with soil microorganisms and significantly influenced by root secretions. In present study we have studied the microflora of Potato fields of Indore district of Madhya Pradesh. The sampling of soil from rhizosphere using a soil corer with a diameter of 3 cm at a depth of 0–20 cm and sampling of plant root materials as rhizoplane were collected three times: during seedling stage, peak of vegetative growth and fruiting of potato (October/November to February/March) from potato fields of Indore district of Madhya Pradesh. The samples were serial diluted and streaked over the Nutrient Agar and PDA media Plates for the isolation of Bacterial and Fungus. For the identification of Cultured bacterial and fungal species were further analyzed by morphological and Molecular analysis using PCR and sequencing of 16S and ITS region of Bacteria and Fungus species. We reported various types of bacterial (Pseudomonas fluorescens, Pseudomonas aerogenosa, Bacillus subtilis, Pseudomonas putida etc.) and fungal species (Species of Penicillium and Trichoderma etc.) which are required for the development of healthy plant and protection of potato plant from other pathogens.
 Keywords: Bacterial species, Fungal species, Molecular identification, 16S Gene.