The isolation and identification of cadmium-resistant Brevibacillus agri C15

Abstract

Eight Cd-resistant bacteria were isolated from the soil. Based on the resistance and specific growth rate, one isolate from these was chosen. The 16S rRNA (rrs) gene sequence was used to assert this isolate belongs to the genus of Brevibacillus and closely related to Brevibacillus agri (B.agri) DSM 6348T (AB112716). The isolate was termed B.agri C15. The specific growth yields and the Cd-dependent fall in specific growth yield of B.agri C15 was determined under different concentrations of Cd. The current study provides a basis for isolation Cd resistant bacteria with maximum tolerable concentration (MTC) 15 mM Cd from a contaminated soil 490±50 μmolal Cd that could be suitable for a new Cd-bioremediation process; therefore, further studies are needed to investigate this possibility.