Abstract
The plasma membrane ofHalobacterium salinarium, strain 1, has been isolated and characterised. A fraction containing cell envelope vesicles was isolated from a cell homogenate by centrifuging. A crude membrane fraction was obtained from the envelope fraction by dialysing it against distilled water, incubating with nucleases and centrifuging. A nucleotide-free purified membrane fraction, identified with the plasma membrane, was obtained by gel-filtration chromatography of the crude membrane fraction on Agarose.
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