Abstract
This chapter discusses the isolated mitotic apparatus (MA) and chromosome motion. Formation of the MA and of the contractile ring are two important events in cell division in the sea-urchin egg. The MA consists of a framework of microtubules (MTs) functioning in the mitotic movement of daughter chromosomes and the contractile ring may be a device for cleavage. Recent advances in studies on the structure and function of MTs and contractile systems in non-muscle cells have provided insights into molecular events taking place in the mitotic process and cleavage of the sea-urchin egg. Sea-urchin egg tubulin has a relatively low affinity for colchicine. Attempts to polymerize tubulin in egg extracts have not succeeded because of the low amount of tubulin and the presence of inhibitory material in marine eggs.
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