Abstract
SUMMARY: Medium for growth of Agrobacterium tumefaciens was made iron-deficient by passage through Seitz filter pads, which removed up to 3·2 mg. Fe3+ from aqueous FeCl3 solutions. The growth rate of A. tumefaciens in this medium was retarded, no 3-ketosucrose accumulated in the culture supernatant and no glucoside 3-dehydrogenase activity was detected in the cells. Addition of Fe3+ to the filtered medium restored the growth rate, glucoside 3-dehydrogenase activity and 3-ketosucrose production. The optimum iron concentrations for maximum glucoside 3-dehydrogenase activity (1 to 3 mg. Fe2+/l.) and yield of 3-ketosucrose (0·5 mg. Fe2+/l.) were considerably less than the minimum requirement of 15 mg. Fe2+/1. for the maximum growth rate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
