The ionic strength of the intermembrane space of intact mitochondria is not affected by the pH or volume of the intermembrane space

Abstract

Ionic strength affects the electron transport activity of cytochrome c through its electrostatic interactions with redox partners and membrane lipids. We previously reported (Cortese, J.D., Voglino, A.L. and Hackenbrock, C.R. (1991) J. Cell Biol. 113, 1331–1340) that the ionic strength (I) of the intermembrane space (IMS-1) in isolated, intact condensed mitochondria is similar to the external, bulk I, over a wide range of bulk I. We now consider the possible effects of IMS-pH and IMS-volume, both variable parameters of mitochondrial function in situ, on IMS-1 IMS-pH and IMS-1 were measured with pH- and I-sensitive fluorescent probes (highly fluorescent FITC-dextran for IMS-pH and FITC-BSA for IMS-1). These probes were delivered into the IMS of intact mitochondria via probe encapsulation into asolectin vesicles, followed by low pH-induced fusion of the vesicles with the outer membranes of intact mitochondria. IMS-pH was found to be 0.4–0.5 units lower than bulk pH over the pH range 6.0–8.5 for mitochondria with a large IMS-volume separating the two mitochondrial membranes (condensed configuration), and 0–0.2 units lower for mitochondria with a small IMS-volume and membranes closely opposed (orthodox configuration). This small pH difference between IMS-pM and bulk pH did not influence the similarity between IMS-1 and bulk I. When the IMS-volume was osmotically decreased, bringing the two mitochondrial membranes in close proximity as in the orthodox configuration. IMS-1 followed the bulk I above 10 mM but did not respond to changes in bulk I below 10 mM. The lack of response of the IMS-1 below 10 mM indicates that the close proximity of the two mitochondrial membranes excludes ions only at low, nonphysiological I. Since the similarity of IMS-1 and bulk I is unaffected by either IMS pH or IMS-volume above a bulk I of 10 mM, at cytosolic physiological I (i.e., 100–150 mM) cytochrome c can be expected to be a free, three-dimensional diffusant in the IMS irrespective of the pH or volume of the IMS.