Abstract
The anti-inflammatory drugs tolfenamic acid, mefenamic acid and nimesulide were investigated for their in-vitro effect on lipid peroxidation, hydroxyl and superoxide anion radicals and for their interaction with 1,1-diphenyl-2-picrylhydrazyl-stable free radical (DPPH). Lipid peroxidation of rat hepatic microsomal membranes was induced by Fe2+/ascorbic acid and assessed spectroscopically as the 2-thiobarbituric acid reactive material. The competition of these agents with dimethylsulphoxide for hydroxyl radicals generated from Fe3+/ascorbate was determined and the second-order rate constants for this reaction were calculated. Their superoxide anion radical quenching ability was assessed using the xanthine/xanthine oxidase system. It was found that tolfenamic and mefenamic acids inhibited significantly lipid peroxidation, while nimesulide had no effect. All three compounds were potent hydroxyl-radical scavengers, but they could not interact with DPPH. Tolfenamic and mefenamic acids demonstrated considerable, and nimesulide moderate superoxide anion radical-quenching activity. The results correlated with the structure and lipophilicity of the examined compounds. It is suggested that the antioxidant properties of these agents may contribute to their anti-inflammatory action.
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