The Involvement of the hsa_circ_0088494-miR-876-3p-CTNNB1/CCND1 Axis in Carcinogenesis and Progression of Papillary Thyroid Carcinoma.

Weiyang Lou,Yongfang Xu,Jiannan Wang,Bisha Ding

doi:10.3389/fcell.2020.605940

Weiyang Lou, Yongfang Xu + Show 2 more

Open Access

https://doi.org/10.3389/fcell.2020.605940

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Abstract

Recently, growing studies have demonstrated that circular RNAs (circRNAs) function as critical players in multiple human tumors, including papillary thyroid carcinoma (PTC). However, the expression and underlying potential mechanism of circRNAs in PTC are still not fully elucidated. In this study, 14 candidate differentially expressed circRNAs (DECs) between normal thyroid tissues and benign thyroid tissues or PTC were first screened using the GSE93522 dataset by the GEO2R online tool. Then, the structural loop graphs of these 14 circRNAs were obtained through the CSCD database. After performing miRNA co-prediction by combination of CSCD and CRI databases, a potential circRNA-miRNA sub-network, consisting of 9 circRNAs and 21 miRNAs, was successfully constructed. Subsequently, the expression and prognostic values of these miRNAs were further determined by starBase, and two miRNAs, namely, miR-605-5p and miR-876-3p, were identified as key miRNAs in PTC. Then, their downstream target genes were predicted by the miRNet database. CTNNB1 and CCND1 were found to be two most potential targets of miR-876-3p by combination of multiple in silico analyses, including protein–protein interaction (PPI), hub gene screening, correlation analysis, and expression analysis. Conclusively, we established a key hsa_circ_0088494-miR-876-3p-CTNNB1/CCND1 axis linked to carcinogenesis and progression of PTC, which may provide promising therapeutic targets in treating PTC in the future.

Highlights

Papillary thyroid carcinoma (PTC) is one of the most prevalent malignant tumors all over the world, which accounts for more than 80% of thyroid cancers (Lim et al, 2017)
It is of great significance to uncover the underlying molecular mechanism of papillary thyroid carcinoma (PTC) carcinogenesis and progression, which may provide novel therapeutic targets for PTC treatment
Increasing evidences have showed that deregulation of circRNAs widely appears in a series of human diseases and dysregulated circRNAs play key roles in pathogenesis of these diseases, especially cancers (Ding et al, 2020; Guo et al, 2020; Yang T. et al, 2020)

Summary

Introduction

Papillary thyroid carcinoma (PTC) is one of the most prevalent malignant tumors all over the world, which accounts for more than 80% of thyroid cancers (Lim et al, 2017). Among all malignant cancer types, PTC’s prognosis is generally good and the 10-year survival rate of PTC has reached about 90% due to its low rate of tumor growth and high degree of tumor differentiation hsa_circ_0088494-miR-876-3p-CTNNB1/CCND1 Axis in Papillary Thyroid Carcinoma (Gui et al, 2020). Wang et al (2018) suggested that circ-ITCH inhibited progression of PTC through the miR22-3p/CBL/beta-catenin pathway; Bi et al (2018) showed that circRNA_102171 promoted the progression of PTC by regulating CTNNB1P1-dependent activation of the beta-catenin pathway; Liu et al (2018) indicated that hsa_circ_0060060

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