Abstract
Activation of PKC by PMA was shown to promote the separation of chicken cardiomyocytes from each other in culture. Immunofluorescence staining for N-cadherin indicated that PMA, but not its inactive isoform 4αPMA, induces the separation of cardiomyocytes and of co-cultured fibroblasts at intercellular junctional regions. The PMA-induced separation of cardiomyocytes and of co-cultured fibroblasts was inhibited by the PKC inhibitor, H-7. Immunoblot analysis further demonstrated that both PKCι and PKCλ were expressed in the cardiomyocyte cultures. While PKCλ was localized to the cell–cell contact areas between cardiomyocytes, PKCι was only detectable in the perinuclear cytoplasm of the co-cultured fibroblasts. The present findings suggest the involvement of PKC in regulating N-cadherin-mediated adherens junction formation in chicken cardiomyocytes. The differential distribution of PKCλ and PKCι in the cardiomyocytes and in the co-cultured fibroblasts suggests that different PKC isozymes are involved in regulating the assembly of intercellular junctions in these two cell types.
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