The involvement of central cholinergic mechanisms in cardiovascular responses to intracerebroventricular and intravenous administration of thyrotropin-releasing hormone

Abstract

Intracerebroventricular (i.c.v.) administration of thyrotropin-releasing hormone (TRH) in a range from 0.1 to 100 μg induced a dose-related increase in blood pressure in conscious rats, whereas TRH-free acid (TRH-OH) and histidyl-proline diketopiperazine (His-Pro-DKP), metabolites of TRH, did not. The blood pressure responses to intravenous (i.v.) injection of 5mg/Kg TRH were similar to those induced by TRH (i.c.v.). Pretreatment with atropine (50 μg, i.c.v.) significantly reduced the pressor effect of TRH administered through either route. Hemicholinium-3 (50 μg, i.c.v.), an inhibitor of choline uptake, also prevented the increase in blood pressure induced by TRH (10 μg, i.c.v.). These results indicate that both centrally and peripherally administered TRH have pressor effects that are mediated by central cholinergic mechanisms, probably by activating cholinergic neurons.