Abstract
Study of the acid-based induced fluorescence transient, the so-called ‘reverse electron flow’, in chloroplasts revealed the following new properties: 1. (1) Experiments in which the acid-base transition was performed in the absence of the measuring beam showed that the high fluorescence state induced by a pH-jump was attained and decayed even in complete darkness. These results indicated that a non-photochemical electron transfer was affected by the pH transition. A pH-induced reverse electron transfer from some secondary electron acceptor to Q probably occurred during that process. 2. (2) This conclusion was supported by the effect of the Photosystem I electron acceptor methyl viologen. Methyl viologen accelerated the decay phase of the transient showing that this phase was controlled by the rate of electron flow to Photosystem I, but this acceptor did not diminish the size of the transient's initial rise, probably because this rise reflected a pH effect on a non-photochemical step located between Q and P-700. 3. (3) The size of the fluorescence transient was dependent upon the reduction state of both parts of the secondary pool of electron acceptors, A 2 and A 1. 4. (4) Redox potential measurements using ferricyanide-ferrocyanide mixtures showed that the size of the transient was directly dependent on a midpoint potential of +385 mV at pH 6.9 and with n = 1. This suggested the involvement of a high potential secondary electron acceptor in the acid-base induced reduction of Q. 5. (5) The restoration of the acid-base transient in long-time dark-adapted chloroplasts was demonstrated by using additional dark incubation with appropriate couples of reductants and lipophilic mediators. Most significant was the restoration of the full size of the transient by the couple ascorbate + diaminodurene which poised a potential of +100 mV, a potential at which the A 2 pool of 5 equivalents stayed completely oxidized in the dark. I conclude that the source of reducing equivalents for the ‘reverse reduction of Q’ was either in the high potential pool A 1 at the level of cytochrome f and plastocyanin or in a similarly high-potential side component.
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