Abstract
A unique low molecular weight antigen fragment-oligoribonucleopeptide conjugates that was isolated from the spleens and lymph nodes at 5 hr after the intravenous injection of antigen, S 35-BSA, produced the anamnestic response 4 days after the injection into rabbits which were previously immunized with BSA. These lymphoid conjugates after incubation with the specific BSA antibodies formed radioactive precipitin arcs when subjected to radioimmunoelectrophoresis against goat anti-rabbit immunoglobulin G. These data suggest that the antigen fragments which conjugate with the oligoribonucleopeptides were the antigenic determinants derived from the BSA molecule. The immunogenicity of the conjugates was shown in normal rabbits 3 days after the injection: that is, detecting the antibodies to S-BSA in the concentrated immunoglobulin G fraction of sera by radioimmunodiffusion and the antibody-forming cells in spleens by the liquid plaque technique. In view of the chemical properties of these conjugates, their capacities for inducing antibody formation suggests that they may be the fundamental antibody inducer.
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