Abstract
Objective To clarify the inhibitory effect of Tripterine combined with Cisplatin on C6 glioma cells and its apoptosis mechanism. Methods C6 glioma cells were treated with Tripterine, Cisplatin, or Tripterine combined with Cisplatin. CCK-8 assay was used to detect the growth inhibition rate in each group. Flow cytometry analysis was used to test the cell apoptosis rate. The expressions of apoptosis-related proteins including Bcl-2, Bax, XIAP, NF-kB were analyzed by ELISA. Results Compared with Tripterine- or Cisplatin-treated group, the inhibition ratio of cell growth in Tripterine and Cisplatin combination group was (69.76±7.28)%, which could significantly inhibit the growth of C6 glioma cells(t=23.78, P<0.01). The apoptosis rate was significantly higher(47.75±5.63)%in combination group than in Tripterine or Cisplatin treated group. The results of ELISA showed that the expression of Bax was significantly higher and Bcl-2, XIAP, NF-κB were obviously lower in the combination group than in Tripterine or Cisplatin treated group (t=35.27, P<0.01). Conclusion The combination of Tripterine and Cisplatin significantly increases the inhibition rate on C6 glioma cells through upregulating Bax and inhibiting Bcl, XIAP and NF-kB to induce the apoptosis of C6 glioma cells. Key words: 【Keywords】 Tripterine; Cisplatin; C6 glioma cell; Apoptosis
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