The investigation of miR-196a2 rs11614913 with breast cancer susceptibility in south of IRAN

Abstract

BackgroundRecent research has revealed the substantial role of MicroRNAs as a hallmark of some diseases such as cancer. These molecules belong to a unique category of endogenous, small non-coding RNAs which can have an effect on regulating gene expression by degrading mRNAs or suppressing translation. They play an important role as regulators of tumor suppressors and oncogenes in pathological processes. Single Nucleotide Polymorphisms (SNP) which is located in the miRNAcoding genes may contribute to the process of the development of various cancers, including breast cancer. This process occurs by altering the expression of mature miRNA. Former studies have observed the association of miRNA SNPs with susceptibility to breast cancer. ObjectiveOur study evaluated the effect of miR196-a2 polymorphism (rs11614913) on patients suffering from breast cancer in south of Iran. Methods and resultsIn this research, the hsa-miR-196a2 rs11614913 SNP was examined in 200 Iranian women including 100 patients suffering from breast cancer and 100 healthy women. The SNP was determined by RFLP-PCR. The X2 or Fisher's exact test was used to compare the control and the experimental groups. Statistical analysis of SNPs frequencies showed no significant difference among the patients and the control group regarding the distribution of genotype (P = 0.831). ConclusionsThis study's results indicated that the TT polymorphic genotype in hsa-miR-196a2 rs11614913 SNP was not related to breast cancer risk among Southern Iranian patients.