The Investigation of Lectin-Like Oxidized LDL Receptor 1 (LOX-1) K167N Polymorphism, Inflammation, and Lipid Status in Patients with Coronary Artery Bypass Grafting

Abstract

Objective: Coronary artery disease (CAD) is a pathological condition resulting from atherosclerosis in the coronary arteries. Besides traditional risk factors, genetic factors such as single nucleotide polymorphs (SNPs) can be involve in disease process. Inflammation plays a role in pathological changes throughout atherosclerosis, from initiation to progress. In this study, we aimed to evaluate the effects of lectin-like oxidized LDL receptor 1 (LOX-1) K167N polymorphism, inflammation, and lipid status in patients with coronary artery bypass grafting (CABG). Material and Methods: The study population consisted of 129 CAD patients who had undergone CABG, and 71 healthy controls. The LOX-1 K167N polymorphism was genotyped using PCR-RFLP technique. Plasma interleukin-1β (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin-33 (IL-33) levels were determined by enzyme-linked immunosorbent assay (ELISA) kits. Results: The distribution of the LOX-1 K167N genotypes and alleles did not differ significantly between CAD patients with CABG and controls. There were no significant differences in plasma IL-1β, TNF- α, IL-33, HDL-C, and LDL-C levels between the patients and controls. IL-1β and systolic blood pressure values were significantly higher in KK genotype patients compared to the same genotype controls(P<0.05). Similarly, systolic blood pressures were higher in NK genotype patients compared with NK genotype controls (P<0.01). Conclusions: IL-1β and systolic blood pressure values were found to be higher in post CABG CAD patients with the KK genotype compared to healthy controls with the same genotype while inflammatory markers and lipid profiles selected according to LOX-1 K167N polymorphism genotype and allele distributions did not differ between groups. Although we couldn’t find an assocation between LOX-1 K167N polymorphism and CAD other than high BP in our study, studies with larger sample sizes might reveal such a relation.