The investigation of etiology for the acute respiratory tract viral infection in children under 7 yearn old

Abstract

Objective To study etiological characteristics of common respiratory viruses infection in children under 7 years old in Dongguan city of Guangdong province.Methods The total RNA were extracted from 340 specimens which had been collected from children under 7 years old during February 2007 to October 2007 in Shijie hospital.During the study the respiratory viruses including respiratory syncytial virus A and B(RSV-A and B), influenza A and B(FLU-A and B), parainfluenza 1, 2, 3(PIV-1, 2 and 3) and Human metapneumo virus (hMPV) were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The common respiratory viruses were demonstrated in 340 children as the positive 50.29%(171/340),including 69 cases (20.29%) with RSV-A infection, 39 cases(11.47%) with FLU-A, 32 cases (9.41%) with hMPV, 22 cases (6. 47%) with PIV-3,9 cases (2.65%) with RSV-A, and 16 cases (9.36% of all viral infection positive cases) with the mixed infection.In these respiratory viruses mixed infection cases,there were 5 hMPV cases coinfected with RSV-A, 4 RSV-A cases with PIV-3, 2 hMPV eases with PIV-3, 2 hMPV cases with FLU-B, 2 cases FLU-A with PIV-3, and 1 ease RSV-A with FLU-A, FLU-B, PIV-1, PIV-2, and multiple mixed infections haven't been detected in this study.Conclusions The data show common respiratory viruses are significant pathogens in acute respiratory infection of paediatric patients under 7 years old in Dongguan city.RSV is still the leading causes of acute respiratory infection in children, the next pathogens respectively are FLU-A and hMPV. Key words: Children; Acute respiratory infection; Respiratory virus; Etiology