Abstract
Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI). Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin. Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method.
Highlights
Group B Streptococcus (GBS) is one of the most causes of neonatal infections
The previous investigation demonstrates that 10-40% of pregnant women have a bacterium colonized and 70-80% of those bacteria transmitted to infants that are colonization and subsequent diseases in the infant which could occur in the uterus at birth or in the first months of life
The aim of the present study was screening for GBS colonization of pregnant women by culture and Colony-PCR methods for BCA gene
Summary
Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. A number of S. agalactia has been resistant at least in one of the antibiotics erythromycin, penicillin, vancomycin, and clindamycin [4]. The aim of the present study was screening for GBS colonization of pregnant women by culture and Colony-PCR methods for BCA gene. Instructions and the use of the antibiotic discs of erythromycin, penicillin, vancomycin, and clindamycin, and concentration, resistance, and sensitivity of these antibiotics were determined.
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