Abstract
The advantages of recombinant enzymes over native include the control in a production environment, product purity and also high yield. The paper presents the determination the optimum temperatures and the activation energies for various origin recombinant exo-inulinases, among others from Aspergillus niger, A. awamori, Kluyveromyces marxianus and K. cicerisporus. The parameters were estimated based on the literature of the activity curves versus temperature for hydrolysis of inulin. It was assumed that both the hydrolysis reaction process and the deactivation process of recombinant exo-inulinase were first-order reactions by the enzyme concentration. A mathematical model describing the effect of temperature on recombinant exo-inulinase activity was used. Based on the comparison analysis, values of the activation energies {E_{rm a }} were in the range from {32.01 pm 7.80} to {43.83 pm 4.87} kJ mol^{-1}, the deactivation energies {E_{rm d }} were in the range from {83.93 pm 4.82} to {352.44 pm 14.26} kJ mol^{-1} and the optimum temperature {T_{rm opt }} were obtained in the range from {318.91 pm } 1.19 to {328.76 pm 0.25} K.
Highlights
Enzymes are characterized, among others by the optimum temperature
The knowledge of the optimum temperature Topt and the activation energy Ea and deactivation energy Ed of the enzyme enables the optimization of the bioprocess and the reduction of its costs at the same time
The highest deactivation energy Ed value was obtained for the fungal recombinant exoinulinase Kluyveromyces marxianus, which together with an endo-inulinase gene from A. niger F4 were cloned and expressed in Y. lipolytica Po1h [20]
Summary
Among others by the optimum temperature. When its exceeded, there is thermal denaturation of enzyme and a rapid decrease in enzyme activity. Keywords Optimum temperature · Recombinant exo-inulinase · Activation energy · Deactivation energy It is necessary to determine the optimum temperature Topt , activation energy Ea and activation energy of the deactivation process Ed for recombinant exo-inulinases.
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