Abstract
Natural resistance-associated macrophage proteins (NRAMPs) have been shown to transport a wide range of divalent metal ions, such as manganese (Mn), cadmium (Cd), and Iron (Fe). Iron is an essential micronutrient for plants and Fe deficiency can lead to chlorosis or decreased biomass. AtNRAMP6 has demonstrated the capability to transport Cd, but its physiological function is currently unclear. This study demonstrates that AtNRAMP6 is localized to the Golgi/trans-Golgi network and plays an important role in intracellular Fe homeostasis in the flowering plant genus Arabidopsis. GUS tissue-specific expression revealed that AtNRAMP6 is highly expressed in the lateral roots and young leaves (three to four top leaves) of Arabidopsis. Moreover, knocking out AtNRAMP6 was shown to impair lateral root growth without having a differential effect on the main root under Fe-deficient conditions. Lastly, the expression of AtNRAMP6 was found to exacerbate the sensitivity of the yeast mutant Δccc1 to an excessive amount of Fe. These findings indicate that AtNRAMP6 plays an important role in the growth of Arabidopsis in Fe-deficient conditions.
Highlights
Iron (Fe) is a vital micronutrient for the survival of all organisms
This study further investigates the function of the full-length AtNRAMP6 protein
AtNRAMP6 was aligned with other Natural resistance-associated macrophage proteins (NRAMPs) proteins, including EcoDMT, AtNRAMP1, AtNRAMP2, AtNRAMP3, AtNRAMP4, OsNRAMP1, and OsNRAMP5
Summary
Iron (Fe) is a vital micronutrient for the survival of all organisms. In plants, Fe is used in cellular respiration, photosynthetic electron transport, biosynthesis of chlorophyll, and various other metabolic functions (Bashir et al, 2016). Fe is abundant in the earth’s crust, Fe availability often limits plant growth due to the low solubility of Fe in aerobic environments (Grotz and Guerinot, 2006). The most obvious consequence of Fe deficiency in plants is chlorosis due to a decrease in chlorophyll content, which significantly affects plant growth, development, and product quality (Bashir et al, 2016). In order to deal with Fe deficiency, plants have evolved a dual mechanism for obtaining Fe from the soil, whereby ferric Fe(III) is taken up as complex with organic compounds in graminaceous species, and ferrous iron (Fe2+) is directly taken up by Fe(II) transporters in the other species (Grotz and Guerinot, 2006). Once Fe has been transported into the cytosol of the plant, it is sent to proteins and organelles for use or storage (Hell and Stephan, 2003)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
