Abstract
We aimed to explore the expression of systemic inflammatory factors and selected intracellular miRNAs that regulate inflammatory signaling pathways potentially involved in age-related macular degeneration (AMD) pathogenesis. A total of 179 patients with wet AMD, 175 with dry AMD and 121 controls were enrolled in the study. Soluble inflammatory factors were analyzed in plasma samples using Luminex technology. Expression of selected miRNAs was analyzed in isolated nucleated peripheral blood cells (PBNCs) using real-time qPCR. Wet AMD was an independent factor associated with higher concentrations of IL-6 (β = +0.24, p = 0.0004), GM-CSF (β = +0.31, p < 0.001), IFN-γ (β = +0.58, p < 0.001), higher expression of miRNA-23a-3p (β = +0.60, p < 0.0001), miRNA-30b (β = +0.32, p < 0.0001), miRNA-191-5p (β = +0.28, p < 0.0001) and lower concentration of IL-1β (β = −0.25, p = 0.0003), IL-5 (β = −0.45, p < 0.001), IL-10 (β = −0.45, p < 0.001), IL-12 (β = −0.35, p < 0.001), lower expression of miRNA-16-5p (β = −0.31, p < 0.0001), miRNA-17-3p (β = −0.18, p = 0.01), miRNA-150-5p (β = −0.18, p = 0.01) and miRNA-155-5p (β = −0.47, p < 0.0001). Multivariate analysis revealed that dry AMD was an independent factor associated with higher concentration of GM-CSF (β = +0.34, p < 0.001), IL-6 (β = +0.13, p = 0.05), higher expression of miRNA-23a-3p (β = +0.60, p < 0.0001), miRNA-126-3p (β = +0.23, p = 0.0005), miRNA-126-5p (β = +0.16, p = 0.01), miRNA 146a (β = +0.14, p = 0.03), and mRNA191-5p (β = +0.15, p = 0.03) and lower concentrations of TNF-α (β = +0.24, p = 0.0004), IL-1β (β = −0.39, p < 0.001), IL-2 (β = −0.20, p = 0.003), IL-5 (β = −0.54, p < 0.001), IL-10 (β = −0.56, p < 0.001), IL-12 (β = −0.51, p < 0.001), lower expression of miRNA-16-5p (β = −0.23, p = 0.0004), miRNA-17-3p (β = −0.20, p = 0.003) and miRNA-17-5p (β = −0.19, p = 0.004). Negative correlations between visual acuity and WBC, lymphocyte count, TNF-α, IL-1 β, IL-2, IL-4, IL-6, IL-10 concentrations and miRNA-191-5p, as well as positive correlations between visual acuity and miRNA-126-3p, -126-5p, and -155-5p PBNCs expression were found in AMD patients. No such correlations were found in the control group. Our results may suggest the role of both intra- and extracellular mechanisms implicated in inflammatory response regulation in multifactorial AMD pathogenesis.
Highlights
Age-related macular degeneration (AMD) is a progressive and degenerative eye disease that is a leading cause of vision loss among the elderly population (Hernandez-Zimbron et al, 2018)
We aimed to explore the expression of systemic inflammatory factors and selected intracellular miRNAs that regulate various inflammatory signaling pathways and processes potentially involved in AMD pathogenesis
There were no significant differences in the body mass index (BMI), mean arterial pressure (MAP), iris color values or work conditions between the groups
Summary
Age-related macular degeneration (AMD) is a progressive and degenerative eye disease that is a leading cause of vision loss among the elderly population (Hernandez-Zimbron et al, 2018). Because of its socioeconomic impact together with the growing incidence and severity of the disease, AMD has become a major challenge in ophthalmology in recent years. Two major clinical subtypes of AMD have been established: dry (atrophic) and wet (exudative, neovascular) AMD. Dry AMD is characterized by drusen accumulation and progressive geographic atrophy of the retinal pigment epithelium (RPE) and retina, whereas wet AMD is described by the growth of largely malformed leaky choroidal vessels into the retina (Feehan et al, 2011). The complex etiology of this disease has been linked to various cellular, biochemical, and molecular events, from which inflammation emerges as crucial in AMD pathogenesis and progression (Nowak, 2006; Kauppinen et al, 2016)
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