Abstract
In all domains of life the replication of DNA is carried out by functionally conserved DNA polymerase. Most DNA polymerases can perform both DNA replication and proofreading (via exonucleolysis) functions. In the cell DNA polymerase operates in a crowded environment, with multiple other proteins binding along DNA. However it is unknown how the binding of such proteins affects the activity of DNA polymerase.In this study, we use T7 DNA polymerase as model system to investigate polymerisation (pol) and exonuclease (exo), activities in the presence of DNA bound proteins as potential roadblocks.
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