Abstract
Abstract The kinetics of acceptance of 20 amino acids by Escherichia coli tRNA was examined after heat treatment with and without magnesium. Beside tRNAtrp, those tRNAs specific for histidine and glutamic acid showed a partial loss of amino acid acceptor activity when heated without magnesium. No loss of activity was observed in the tRNAs of the remaining 17 amino acids. Also examined was tRNAtrp from various bacterial species whose DNA composition ranged from 35 to 72% in their guanine-cytosine content. Five of the nine species of bacteria showed the interconversion of tRNAtrp and the remaining four existed only in the active form.
Highlights
SUMMARYThe kinetics of acceptance of 20 amino acids by Escherichia coli tRNA was examined after heat treatment with and without magnesium
By heating the tRNh at 50” for 10 min in a Tria-II
The remaining components for aminoacylation reaction were added to the tRNA solution in ice
Summary
The kinetics of acceptance of 20 amino acids by Escherichia coli tRNA was examined after heat treatment with and without magnesium. Beside tRNA’*D, those tRNAs specific for histidine and glutamic acid showed a partial loss of amino acid acceptor activity when heated without magnesium. By heating the tRNh at 50” for 10 min in a Tria-II
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