The interactive effects of irradiance and photoperiod on Chara vulgaris L.: concerted responses in morphology, physiology, and reproduction

Abstract

Both the single and interactive effects of irradiance and photoperiod on a worldwide charophyte species, Chara vulgaris L., were investigated in a greenhouse experiment. Under high light intensity, plants exhibited shortened shoot, compact canopy and low Chl a/Caro. In contrast, elongated shoot, expanded canopy and low Chl a/Chl b corresponded to low light intensity. In addition, both ash mass ratio and relative growth rate (RGR) were positively related to light intensity. The effect of photoperiod on plants was relatively complex. Although photoperiod did not affect morphological characters, an increased photoperiod significantly decreased Chl a/Chl b and Chl a/Caro ratios. Two-way analysis of variance (ANOVA) analysis indicated that significant interaction of irradiance and photoperiod was present on ash mass ratio, RGR, Chl a/Chl b, Chl a/Caro and Chl (a + b)/Caro ratios. The high RGR found for 50% sunlight, L:D 16:8 (50/16) conditions along with the significant interaction of irradiance and photoperiod on RGR indicated that the effect of irradiance was more important than photoperiod for plant growth. And finally, both irradiance and photoperiod had positive effects on the emergence of sex organs (♂♀) and the maturation of oospores, except that increased photoperiod did not accelerate the maturation of oospores. In summary, for both plant growth and reproduction, C. vulgaris was able to acclimate morphologically and physiologically to different irradiance levels and photoperiods. This study can partly explain the broad geographic distribution of C. vulgaris.