The interactions of Rose Bengal and other aromatic anionic dyes with mannitol-1-phosphate dehydrogenase fromEscherichia coli

Introduction: Most Oral Squamous Cell Carcinoma (OSCC) is preceded by Oral Potentially Malignant Disorders (OPMDS). These disorders if diagnosed early can be prevented from converting into full blown malignancies. This points towards an ever increasing need for a more accurate, less invasive diagnostic tool to detect these lesions early. Aim: To analyse and compare the accuracy of 1% Rose Bengal (RB) dye and oral microbiopsies (using dermatologic ring curettes) with conventional scalpel biopsy in diagnosing oral epithelial dysplasias. Materials and Methods: This crosssectional pilot study, included a total number of 26 male patients, 40-60 years old, with oral white and red mucosal lesions attending the Outpatient Department (OPD) of Teerthanker Mahaveer Dental College, Moradabad, Uttar Pradesh, India. Thorough clinical examination, 1% RB staining, microbiopsy, and scalpel biopsy was performed on all included participants. Parameters assessed included clinical signs indicative of dysplasia in red and white lesions such as increase thickness, nodularity, atrophic mucosa, erosion,ulcers and change in colour of mucosa with positive history of tobacco smoking. Hyperchromatic areas owing to increase stain intake (due to increased nuclear cytoplasmic ratio) were obtained after staning with RB dye and histopathological indicators of epithelial dysplasia (cellular and architectural changes) were observed after both microbiopsy and scalpel biopsy. Chi-square test was done to compare results of 1% RB staining and microbiopsies with scalpel biopsies. Results: Total 26 male patients were included with a mean age of 50 years. There was no statistically significant difference in the accuracy of microbiopsies (p=0.913) and 1% RB dye (p=0.393) as compared to conventional scalpel biopsy in delineating the epithelial dysplastic changes associated with OPMDS. Conclusion: Oral microbiopsy, is a relatively novel, accurate, and less invasive diagnostic tool. It has proved to be as effective as scalpel biopsy in diagnosing and grading epithelial dysplasia. Through this article, it was proposed that 1% RB staining, microbiopsy, and scalpel biopsy can be used in conjugation as a part of cytology-cum-histopathology based diagnostic scheme for oral clinically suspicious lesions

The powdered seeds of Plantago ovata (PSPO) were utilized for the removal of Malachite Green (MG) and Rose Bengal (RB) dyes from aqueous media by batch adsorption. The Fourier transform infra red spectroscopy (FTIR) results showed that both the dyes were adsorbed between the cellulose matrices, and this has been verified from the intensifying and narrowing aromatic C-H bending vibration. The morphology of the dye laden adsorbent was studied by scanning electron microscopy (SEM), which showed that the dyes were adsorbed between the cellulose matrices of the adsorbent. The PSPO was found to be very effective for the removal of MG and RB at pH 7, and equilibrium was attained within 200 min. The kinetic study indicated that the rate limiting step for MG and RB adsorption may be chemisorption and intraparticle diffusion. Adsorption equilibrium data were fitted to Langmuir, Freundlich, Redlich-Peterson and Temkin adsorption isotherms. It is inferred from the equilibrium studies that the adsorption of MG follows the Freundlich isotherm and the adsorption of RB follows the Langmuir isotherm. The maximum monolayer adsorption capacity of the PSPO was found to be 86.23 mg/g for MG and 81.23 mg/g for RB, respectively.

Adsorption of rose Bengal dye from aqueous solution by amberlite Ira-938 resin: kinetics, isotherms, and thermodynamic studies

Fabrication of biodegradable cellulose acetate nanofibers containing Rose Bengal dye by electrospinning technique and their antiviral efficacy under visible light irradiation

Optimization of process parameters for determination of trace Hazardous dyes from industrial wastewaters based on nanostructures materials under ultrasound energy

In this study, hierarchical calcium phosphate (CaP) nanoflowers were synthesized from cuttlefish bone using a hydrothermal method. A CaP–TiO 2 (1%) nanocomposite was subsequently prepared via an in situ approach while keeping the original CaP framework. The structural, morphological, and compositional properties of CaP and CaP–TiO 2 were characterized using x‐ray diffraction (XRD), dynamic light scattering (DLS) for hydrodynamic size determination (506 nm), BET surface area analysis (82.57 m 2 g − 1 ), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) coupled with EDX, and high‐resolution transmission electron microscopy (HR‐TEM). A sono‐photocatalytic process was employed for the efficient removal of environmental pollutants, namely bisphenol A (BPA) and Rose Bengal (RB) dye. The CaP–TiO 2 nanocomposite exhibited superior degradation efficiencies of 89% for BPA and 83% for RB, compared to pristine CaP, which showed 76% and 69% degradation, respectively. The synergistic effect of ultrasonic irradiation and UV light enhanced reactive radical generation, leading to improved degradation performance. The possible degradation pathways for both BPA and RB dye are also proposed.

ABSTRACTV2O5 nanoparticles were prepared by ultrasound-assisted method and characterized using XRD, FTIR, SEM, and TEM. XRD pattern revealed an orthorhombic V2O5 phase with an average crystallite size of 52 nm. FTIR spectrum indicated stretching vibration of V-O-V at 430 cm−1. The morphology of nanoparticles was determined by SEM and TEM analysis. The optical bandgap of V2O5 nanoparticles was found to be 2.4 eV. The photocatalytic degradation study of V2O5 nanoparticles was investigated against the Rose Bengal (RB) dye under solar light irradiation. Ultrasound-assisted V2O5 nanoparticles showed an excellent photocatalytic activity (RB dye). The antibacterial activity (disc diffusion method) of ultrasound-assisted V2O5 nanoparticles was studied against human pathogenic bacteria (S.aureus, E.coli, P.aeruginosa and P. Vulgaris). The results obtained indicate that the prepared nanoparticles will be useful in dye degradation and biomedical applications.

The present work demonstrates the Green synthesis of silver nano-particles (AgNPs) using the Rhododendron campanulatum flower extract and their potential applications as photo catalyst and antioxidant. Several spectral approaches viz., UV-Vis, XRD, SEM-EDAX, BET isotherm and TEM were used to characterise the biosynthesized AgNPs in detail.The UV-Vis spectroscopy showed that the biosynthesized AgNPs have surface plasmon resonance (SPR) peak at 472 nm. The X-ray diffraction (XRD) studies confirmed the crystalline nature of biosynthesized AgNPs with mean crystalline size ~22.97 nm. According to the TEM examinations, the synthesized AgNPs were poly dispersed, smooth in morphology, irregularly shaped, and had an average grain size of 43.75 nm. The adsorption studies showed that the biosynthesized AgNPs have the single point surface area (at P/Po) was 0.1398 m2/gm, BET surface area was 0.1057 m2/gm, Langmuir surface area was 0.1465 m2/gm, and the micro pore area was 0.7834 m2/gm. The antioxidant efficacy of as synthesized AgNPs was determined using DPPH scavenging method, and the IC50 value was observed 38.68 μg/mL. Photocatalytic activity of synthesized AgNPs was determined by studying the adsorption/desorption equilibrium between the AgNPs and the solutions of MB, and RB dyes. The synthesized AgNPs showed the dye degradation efficacy of 73.88% for methylene blue (MB) dye, and 76.62% for Rose Bengal (RB) dye; however, in the absence of AgNPs, the degradation efficacy was observed as17.55% and 48.80% in 4 hours under photo-irradiation for MB and RB dye, respectively.

Visible light assisted photocatalysis of Methylene Blue and Rose Bengal dyes by iron doped NiO nanoparticles prepared via chemical co-precipitation

Perovskites can absorb solar energy and are extensively used in various catalytic and photocatalytic reactions. However, noble metal particles may enhance the catalytic, photocatalytic, and antibacterial activities. This study demonstrates the cost-effective green synthesis of the photocatalyst perovskite LaMnO3 and its modification with noble metal Ag nanoparticles. The green synthesis of nanocomposite was achieved through a hydrothermal method employing aqueous extract derived from Citrus limon (L.) Burm peels. The properties of fabricated perovskites LaMnO3 and LaMnO3-Ag nanocomposites were evaluated and characterized by Ultraviolet-Visible spectroscopy (UV-Vis), Diffuse Reflectance Spectroscopy (DRS), X-ray diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), High-Resolution Transmission Electron Microscopy (HRTEM), Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDX) and Brunauer–Emmett–Teller (BET) surface area techniques. The particle size distribution % of LaMnO3 and LaMnO3-Ag was observed to be 20 to 60 nm after using TEM images. The maximum percentage size distribution was 37 nm for LaMnO3 and 43 nm for LaMnO3-Ag. In addition, LaMnO3-Ag nanocomposite was utilized as a photocatalyst for the degradation of Rose Bengal (RB) dye and its antibacterial activities against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The surface area and band gap for perovskite LaMnO3 nanoparticles were calculated as 12.642 m2/g and 3.44 eV, respectively. The presence of noble metal and hydrothermal-bio reduction significantly impacted the crystallinity. The BET surface area was found to be 16.209 m2/g, and band gap energy was calculated at 2.94 eV. The LaMnO3 nanocomposite with noble metal shows enhanced photocatalytic effectiveness against RB dye (20 PPM) degradation (92%, R2 = 0.995) with pseudo-first-order chemical kinetics (rate constant, k = 0.05057 min−1) within 50 min due to the ultimate combination of the hydrothermal and bio-reduction technique. The photocatalytic activity of the LaMnO3-Ag nanocomposite was optimized at different reaction times, photocatalyst doses (0.2, 0.4, 0.6, and 0.8 g/L), and various RB dye concentrations (20, 30, 40, and 50 ppm). The antibacterial activities of green synthesized LaMnO3 and LaMnO3-Ag nanoparticles were explored based on colony-forming unit (cfu) reduction and TEM images of bacterial and nanoparticle interactions for S. aureus and E. coli. An amount of 50 µg/mL LaMnO3-Ag nanocomposite was sufficient to work as the highest antibacterial activity for both bacteria. The perovskite LaMnO3-Ag nanocomposite synthesis process is economically and environmentally friendly. Additionally, it has a wide range of effective and exclusive applications for remediating pollutants.

Cellular, histological, and behavioral pathological alterations associated with the mouse model of photothrombotic ischemic stroke

TiO2 nanoparticles were prepared by a microwave-assisted green method using Andrographis paniculata as fuel. XRD, Raman, FESEM with EDS, TEM and UV-DRS have been used for characterization of TiO2 nanoparticles. XRD exhibits a tetragonal structure with the average crystallite size of 19 nm. From Raman studies, the peaks at 196 (Eg), 395 (B1g), 514 (A1g) and 639 (Eg) are revealing the anatase phase of TiO2. FESEM and TEM images confirm the nanoparticles with average particle size are 25 nm. Optical bandgap of biogenic TiO2 nanoparticles is 3.27 eV. Photoluminescence spectrum of TiO2 nanoparticles showed blue and green emissions. The photocatalytic degradation study of biogenic TiO2 nanoparticles was investigated against the Rose Bengal (RB) dye under UV light irradiation. Microwave-assisted green synthesized TiO2 nanoparticles showed an excellent photocatalytic activity (RB dye). Antibacterial activity (disc diffusion method) of microwave-assisted biogenic TiO2 nanoparticles is studied against foodborne pathogens. TiO2 exhibits anti-lung cancer (human lung cancer A549: IC50: 24.96 µg/mL) activity along with biocompatibility.

Facile green synthesis of Cu-doped MoO3 nanoparticles and its application for the photocatalytic degradation of hazardous organic pollutants

Hydrothermally synthesized ZnFe2O4/ZnO heterojunction nanocomposites for enhanced RB dye degradation via Z-scheme photocatalysis

Release, transfer and partition of fluorescent dyes from polymeric nanocarriers to serum proteins monitored by asymmetric flow field-flow fractionation