Abstract
The synthesized cobalt(II) complex, CoPNBDENA and the binding of thiscomplex with sheep genomic DNA were investigated by UV–Visible absorption andviscosity techniques. Also the interaction of sheep genomic DNA with the complex wasstudied using the agarose gel electrophoresis method. The results indicated that thecomplex interacted with DNA. The nature of the binding seemed to be mainly anelectrostatic interaction between DNA and the cobalt(II) complex. Other binding modessuch as hydrogen bonds may also exist in this system. In this study, after the interaction ofDNA– CoPNBDENA, it was observed that the migration of the DNA band became slow asthe amount of cobalt(II) complex was increased. This clearly demonstrates that theCoPNBDENA complex neutralizes the negative charges of DNA.
Highlights
Deoxyribonucleic acid (DNA) plays a significant role in the life process, because it carries the inheritance information and leads the biological synthesis of proteins and enzymes through the replication and transcription of genetic information in living cells
Nair and co-worker [29] worked on the interaction of DNA with cobalt(II) tridentate complex, and the photocleavage studies showed that the cobalt(II) complex increased to nicking of DNA in the presence of plasmid DNA [29]
An electrostatic interaction between CoPNBDENA complex and the DNA can be predicted when it is based on the hyperchromism exhibited and shifted in absorbance of the cobalt complex
Summary
Deoxyribonucleic acid (DNA) plays a significant role in the life process, because it carries the inheritance information and leads the biological synthesis of proteins and enzymes through the replication and transcription of genetic information in living cells. The interaction of DNA with transition metal complexes has got intensive attention in the last few years in order to develop new novel nonradioactive probes of DNA structure [12, 13], new therapeutic agents that cleave DNA [14,15,16] and DNA-mediated electron transfer reactions [17] These complexes give an opportunity to discover the effects of the central metal atom, the ligands and the coordination geometries on the binding event. Cobalt was accepted as an essential metal element widely distributed in the biological systems such as cells and body, and the interaction of DNA with cobalt complex has attracted much attention [26] Their binding properties of cobalt with calf thymus DNA were studied by several methods, and the experimental results showed that the size and shape of the intercalated ligand had an important effect on the binding affinity of the complexes with DNA [27]. Nair and co-worker [29] worked on the interaction of DNA with cobalt(II) tridentate complex, and the photocleavage studies showed that the cobalt(II) complex increased to nicking of DNA in the presence of plasmid DNA [29]
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