Abstract
The electrochemical behavior of the 1-electron couple for the bioreductive drug metronidazole has been examined in the presence and absence of the biological target molecules, DNA bases, and nucleosides, including uracil and uridine. Using cyclic voltammetry as the investigation technique, the change in return-to-forward peak current ratio, ip r ip f , from the control, recorded in the absence of target, was measured as a function of scan rate and biological target concentration. All target molecules, except adenosine and guanine, resulted in interaction with RNO 2 −, as measured by the decrease in the ip r ip f ratio in the following order of increasing reactivity : adenine, guanosine, thymine, uracil, uridine, and thymidine (at a metronidazole:target ratio of 1:1). No decrease in ip r ip f was observed with cytosine or cytidine until ratios of 1:20 and 1:30, respectively, were attained. An approximately linear relationship was found between the percentage change in the CV response and log[target] allowing us to determine the sensitivity of RNO 2 − to the concentration of the target species. The implication for the biological action of metronidazole and other nitro-heterocyclic drugs is discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Radiation Oncology*Biology*Physics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
