Abstract
We have evaluated the binding of sialylated and desialylated lipoproteins to collagen isolated from the proteoglycan and musculoelastic layers of intima and media of uninvolved human aorta and atherosclerotic lesions. Comparing various collagen preparations from the uninvolved intima-media, the binding of sialylated apoB-containing lipoproteins was best to collagen from the intimal PG-rich layer. Binding of sialylated apoB-containing lipoproteins to collagen from this layer of fatty streak and fibroatheroma was 1.4- and 3.1-fold lower, respectively, in comparison with normal intima. Desialylated VLDL versus sialylated one exhibited a greater binding (1.4- to 3.0-fold) to all the collagen preparations examined. Desialylated IDL and LDL showed a higher binding than sialylated ones when collagen from the intimal layers of fibroatheroma was used. Binding of desialylated HDL to collagen from the intimal PG-rich layer of normal tissue, initial lesion, and fatty streak was 1.2- to 2.0-fold higher compared with sialylated HDL.
Highlights
Deposition of extracellular lipids on the components of the connective tissue matrix is one of the earliest and most pronounced manifestations of atherosclerotic lesion in human vessels
Preliminary experiments showed that the binding of both sialylated and desialylated very-low-density lipoprotein (VLDL) to collagen isolated from various layers of the uninvolved aortic intima-media did not depend on collagen concentration, used for coating the wells of microtiter plates, in the range of 1–10 μg/mL
The present results show that circulating lipoproteins can bind to collagens isolated from the PG-rich and musculoelastic layers of the intima and the media of uninvolved and atherosclerotic regions of the human aorta
Summary
Deposition of extracellular lipids on the components of the connective tissue matrix is one of the earliest and most pronounced manifestations of atherosclerotic lesion in human vessels. Data are available for the interaction of lipoproteins, above all of low-density lipoprotein (LDL), with elastin and proteoglycans (PGs) of the vessel wall [1,2,3,4]. Another main component of the extracellular matrix is collagen [5,6,7]. In a number of other studies, LDL binding to different collagen types was examined [17,18,19,20]. Binding of LDL and other human blood lipoproteins to collagen isolated from various layers of the normal and atherosclerotic human aorta has not been studied so far
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