The interaction of phenols, anilines, and benzoic acids with sephadex gels

Abstract

The adsorption by Sephadex dextran gels of monosubstituted phenols, anilines, and benzoic acids can be correlated by the Hammett equation. The interaction of these benzene derivatives with the gel therefore operates through the hydroxyl, amino, and carboxylic groups respectively. It is suggested that the benzene derivatives are adsorbed onto the hydroxyether cross-linking by hydrogen bonds. Halogen-substituted benzene derivatives are exceptions as they are more strongly adsorbed than the Hammett equation predicts. There are indications that the halogen-substituted derivatives are adsorbed by the dextran chains.