The interaction of nuclear and cytoplasmic damage after treatments with toxic chemicals

Abstract

An attempt is made to show how the interaction of different degrees of nuclear and cytoplasmic damage may contribute to the ultimate whole cell damage by a chemical. It suggests that cytoplasmic, as well as nuclear damage, may be important in the action of chemical carcinogens. Using Amoeba proteus as a single cell model where nuclear and cytoplasmic damage can be separated by micrurgy, the mortality curves for the nucleus, the cytoplasm and the whole cell are examined after four different treatments: exposure to N-methyl-N-nitroso urethane, a potent carcinogen in mammalian systems; exposure to ββ 1 (dichlorodiethyl) methyl amine, an alkylating agent used in chemotherapy; exposure to methylmercury chloride, a very toxic organo-metal; and irradiation with X-rays. These illustrate how different relative nuclear/cytoplasmic sensitivities contribute to the death of the cell. The evidence for nuclear and cytoplasmic damage after treatment with the N-methyl-N-nitroso urethane is detailed, and possibilities of nuclear repair after the four different types of treatment examined. Work on Amoeba proteus makes no attempt to assess separately changes in structure or activity of any one of the cells many enzyme systems, but looks at the balance between nuclear and cytoplasmic damage as a whole.