Abstract
One tetracyclic antidepressant, mianserin hydrochloride (MIA), has quite significant side effects on a patients’ health. Cyclodextrins, which are most commonly used to reduce the undesirable features of contained drugs within their hydrophobic interior, also have the potential to alter the toxic behavior of the drug. The present paper contains investigations and the characteristics of interaction mechanisms for MIA and the heptakis (2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) system, and evaluated the effects of the complexation on MIA cytotoxicity. In order to assess whether there was an interaction between MIA and DM-β-CD molecules, isothermal titration calorimetry (ITC) have been chosen. Electrospray ionization mass spectrometry (ESI-MS) helped to establish the complex stoichiometry, and circular dichroism spectroscopy was used to describe the process of complex formation. In order to make a wider interpretative perspective, the molecular docking results have been performed. The viability of Chinese hamster cells were investigated in the presence of DM-β-CD and its complexes with MIA in order to estimate the cytotoxicity of the drug and the conjugate with the chosen cyclodextrin. The viability of B14 cells treated with MIA+DM-β-CD is lower (the toxicity is higher) than with MIA alone, and no protective effects have been observed for complexes of MIA with DM-β-CD in any ratio.
Highlights
Antidepressants were prescribed very often before SARS-CoV-2 [1], and during the COVID-19 pandemic, the usage increased [2]
We mostly examined the physicochemical properties of mianserin hydrochloride during interaction with heptakis (2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) by an isothermal titration calorimetry (ITC), electrospray ionization mass spectrometry (ESI-MS), and circular dichroism spectroscopy (CD) to see how the properties of the drug may change when methyl groups appear in native β-CD
In order to assess whether there was an interaction between mianserin hydrochloride and heptakis (2,6-di-O-methyl)-β-cyclodextrin molecules, isothermal titration calorimetry (ITC) has been chosen
Summary
Antidepressants were prescribed very often before SARS-CoV-2 [1], and during the COVID-19 pandemic, the usage increased [2].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
