Abstract
The aim of the investigations was to measure the influence of Fe3+ ion on jack bean urease (JBU) activity. Interaction between Fe3+ and JBU, is examined using isothermal titration calorimetry. It was found that Fe3+ ions acted as a non-cooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for Fe3+ ions. The small structural parameters show that there are little changes on the JBU structure, indicating that Fe3+ has minor effect on the JBU activity. The association equilibrium constant is 42,484.13 ± 110 mol l−1, indicating the moderate interaction of Fe3+ ion with JBU. The molar enthalpy of binding is ΔH = −4.33 kJ mol−1.
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