Abstract

The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg ++ and Cu ++ interact strongly with tris, and Co ++, Zn ++, Cd ++ much less so. The interaction of tris with Mg ++ and Mn ++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

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