Abstract
The interaction of acridine orange (AO) and deoxyribonucleic acid (DNA) in acetate, citrate, and phosphate buffers between pH 3.15 and 7.0 was studied spectrophotometrically and fluorometrically. The absorbancy of the dye was decreased and the fluorescence quenched in the presence of DNA, and further addition of DNA caused a shift of the absorption maximum to longer wavelengths and an enhancement of fluorescence. The point of wavelength shift in absorbancy and maximum quenching of fluorescence occurred at the same weight concentration of DNA, namely 10 −2 mg. DNA/ml. of reaction mixture. These results are interpreted as indicating the formation of two distinct complexes between DNA and acridine orange.
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