The Interaction of Colloid–Liposome–Protein Ternary Complex with Biological Membrane

Abstract

The formation and properties of complexes obtained via binding of anionic spherical bilayer vesicles (liposomes) on the surface of a cationic carrier—polystyrene microparticles with grafted cationic chains (spherical polycationic brushes)—have been studied. The limiting amount of adsorbed liposomes depends on the molar fraction of the anionic lipid in the liposome membrane ν and decreases from 49 for liposomes with ν = 0.1 to 25 for liposomes with ν = 0.3. The complexes with the ultimate number of liposomes (saturated) carry negative charges, but they are capable of additional adsorption of a significant amount of negatively charged protein (albumin). Brush–liposome–protein ternary complexes can interact with negatively charged borosilicate microspheres covered with lipid bilayer. The obtained results are of interest for understanding the behavior of multiliposome drug carriers in the biological environment, in particular, for predicting their interaction with cells.