Abstract
A very accurate pH stat method was used to measure the number of protons which are taken up when 2,3-diphosphoglycerate (DPG) reacts with deoxyhemoglobin (Hb) and oxyhemoglobin (HbO2). As judged by this method it appeared that the effect of DPG on both Hb and HbO2 is pH dependent with this difference that similar results are obtained at different pH ranges. The data showed that the additional Bohr effect and therefore the oxygen affinity of hemoglobin in the presence of DPG is not only due to the binding of DPG to Hb, but also due to the interaction of DPG with HbO2.
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