The interaction between the 33 kDa manganese-stabilising protein and the D 1/D 2cytochrome b-559 complex

Abstract

Using affinity chromatography with the extrinsic 33 kDa protein as the immobilised ligand, it was demonstrated that the reaction centre complex of photosystem II, composed of the D 1, D 2 and cytochrome b-559 polypeptides, can directly interact with the 33 kDa protein. By this approach it was possible to purify the reaction centre from solubilised photosystem II core complexes since neither the 47 kDa nor the 43 kDa protein would bind to the ligand.