The Interaction Between GDF9 Gene Polymorphism and Age Groups on the in Vitro Maturation of Awassi Sheep Oocytes

Abstract

The study was conducted in the graduate laboratory of the Department of Animal Production, College of Agriculture, Al-Muthanna University, from 20/11/2022 to 22/3/2023, it included the collection of ovaries from the female reproductive organs of sheep slaughtered in the Samawa massacre, transferring them immediately after slaughter to the laboratory for the purpose of extracting Oocytes from the ovaries, subject to laboratory maturing using culture media for mature, Molecular investigations were completed at the Marshes Research Laboratory of Thi Qar University, for the purpose of isolation and purification of DNA, with the aim of finding genetic formations and the relationship between genotypes, morphological characteristics, and the ability to mature in the Awassi sheep Oocytes. Oocytes of Awassi sheep were collected by Oocyte aspiration method, then they were classified and examined for their vitality. The results indicated that there were no significant differences between animals carrying the three genotypes resulting from this mutation at position 205 of the studied segment of the GDF9 gene with damaged, immature and mature Oocytes, with no significant effect between the age groups of animals carrying the three genotypes resulting from this mutation at position 205 of the studied segment of the GDF9 gene with damaged, immature and mature Oocytes.